mRNA Maturation in Giant Viruses: Variation on a Theme

Priet S, et al., 20;43(7):3776-88, Nucleic Acids Res, 2015

This investigation was aimed at identifying the Mimivirus and M. chilensis predicted viral polyA polymerases (vPAPs) with an ability to perform polyadenylation. Based on a series of biochemical and structure biology investigations, the vPAPs are found to be homodimeric and self-processive. A Pall ForteBio BLItz system equipped with Ni-NTA Biosensor probes was used in the binding experiments. His tagged versions of proteins Mg18 or R341 were captured onto Ni-NTA biosensors followed by screening against dilution series of Mg561 or Mg18, respectively.

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