Molecular Basis of FIR-Mediated c-myc Transciptional Control

Cukier, C.D., et al., 17(9), 1058 - 1064, Nature Structural and Molecular Biology, 2010

This publication investigates components of the far upstream element (FUSE) transcriptional control system. Molecular interaction experiments on the Octet RED platform utilized a biotinylated 40-mer of single-stranded FUSE DNA immobilized on Streptavidin biosensors. Affinity constants and association/dissociation rate constants were determined for various DNA binding domains, alone and in combinations: FBP Nbox-KH1-KH4, FBP KH1-KH4,FBP3 Nbox-KH1-KH4, and FIR RRM1-RRM2.

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