Microbial Strain Development Using BioLayer Interferometry

Cantin G, 12(4):54-57, BioProcess International, 2014

The author described a case study for rapid screening of high-producing production strains for expression of granulocyte colony stimulating factor (G-CSF) clones in E. Coli. The Octet platform was used to efficiently screen and quantitate the binding characteristics of the expression clones. Streptavidin biosensors were used. BLI can be used for rapid protein measurement, titer determination, as well as functional analysis applications for a diverse variety of protein types.

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