Maturation and Diversity of the VRC01-Antibody Lineage Over 15 Years of Chronic HIV-1 Infection

Wu X, et al., 161(3):470-85, Cell , 2015

An investigation of the VRC01-antibody lineage that targets the site of CD4 engagement on HIV-1 is reported. Although germline-reverted variants of VRC01-class antibodies were reported to have no binding affinity to gp120, the current investigation provides evidence of binding to the early autologous gp120 molecule, d45-01dG5. A Pall ForteBio Octet RED384 system equipped with Anti-Human IgG probes was used to measure binding constants of HIV-1 gp120 extended core and the germline-reverted antibodies. The affinities of 1995 and 2008 antibodies showed improvements in the nanomolar range. Structure data suggest mutations in 20 amino acids are responsible for the affinity maturation.

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