Mapping the HLA-DO/HLA-DM Complex by FRET and Mutagenesis

Yoon T, et al., 109(28), 11276-81, Proc Natl Acad Sci USA, 2012

The authors characterize a putative binding surface and an overall orientation for HLA-DO inhibition of HLA-DM, two key molecules in the class II presentation pathway. HLA-DO/HAL-DM complexes were analyzed by FRET and mutagenesis, resulting in identification of an apparent binding site. Stable mutant HLA-DO proteins were produced by mutation for these studies. The Octet system and Streptavidin biosensors were used to determine KD values of mutant HLA-DO proteins binding to HLA-DM.

Read More