Label-Free Kinetic Analysis of an Antibody-Antigen Interaction Using Bio-Layer Interferometry

Kumaraswamy S, Tobias R, 1278:165-82, Methods Mol Biol, 2015

This is a book chapter describing the fundamentals of BLI technology, kinetic characterization, assay design considerations, assay development, data analysis, and key factors to keep in mind for a successful antibody-antigen assay development effort. It provides a quick-start guide for life science researchers on kinetic characterizations performed using the BLI platform.

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