Publications

Kinetic Titration Series with Biolayer Interferometry

Frenzel D, Willbold D., 9(9):e106882, PLoS One, 2014

The article describes execution of a strategy often used in surface plasmon resonance (SPR) known as "Kinetic Titration Series" in a BLI platform, and compares it to the "Parallel Sensor Kinetics" method. In SPR, the Kinetic Titration Series involve sequential injections of analyte on a ligand coated sensor chip without observing a complete dissociation between injections. Application of this methodology using the BLI platform described in this report utilize two pairs of interactions, namely IgG binding to protein G B1 and scFv IC16 binding to amyloid β(1-42). Authors compared the data obtained from Kinetic Titration Series and Parallel Sensor Kinetics for the above two systems using the BLI. The protein G B1 immobilized via amine coupling onto AR2G sensors and the biotinylated Aβ(1-42) immobilized onto SSA sensors were used as ligands. The Binding curves were obtained on an Octet RED96 system. According to the report, the execution of Kinetic Titration Series is relatively straightforward and avoids data evaluation discrepancies associated with sensor heterogeneity. This approach could also minimize the number of sensors and material required for kinetic titration assays. However, the caveat of this approach is that the users have to export the data obtained from this assay format into a BiaEvaluation software package that usually comes with the Biacore SPR system.

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