Kinetic Characterization of Salmonella FliK-FlhB Interactions Demonstrates Complexity of the Type III Secretion Substrate-Specificity Switch

Morris, D.P., et al., 49(30), 6386-6393, Biochemistry, 2010

In this paper, FliK-FlhB interactions were characterized in Salmonella using wild-type and two variant FlhBs from mutants with severe flagellar structural defects. The Octet system was used for kinetic analysis; biotinylated FliK was immobilized on Streptavidin biosensors and associated with FlhB. The Octet platform data show a rapid association followed by a slow conformational change and dissociation leading to a complex binding mechanism between FliK-FlhBc.

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