Kinetic Analysis of Antibodies from Different Cultured Media

Ho D, Fletcher, T, and Ni, J, July: 2-5, BioPharm International , 2013

In this study, an Octet QKe system method was established for assessing the effects of media condition on the quality and activity of cultured antibodies. Affinities and binding kinetics of monoclonal antibodies produced from a hybridoma cell line cultured in two different types of medium were measured. With this method, the authors were able to measure the intermolecular binding interactions between the antibodies and targeted protein. Anti-Mouse IgG Fc Capture (AMC) biosensors were used for antibody immobilization. Binding studies with fibronectin used Streptavidin biosensors loaded with biotinylated fibronectin.

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