Key Mutations Stabilize Antigen-Binding Conformation During Affinity Maturation of a Broadly Neutralizing Influenza Antibody Lineage

Xu H, et al., 83(4):771-80, Proteins, 2015

The mechanism of affinity gain in a human B-cell lineage was investigated in which, three mature antibodies with the ability to neutralize a broad range of H1 influenza viruses have been used. Kinetic measurements obtained using a Pall ForteBio BLItz system involves Fab immobilization on to Ni-NTA Biosensor probes. The association and dissociation of varying concentrations of H1 influenza hemagglutinin (HASI) head molecules against different Fab variants yielded the kinetic parameters.

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