Intrinsic Selectivity of Notch 1 for Delta-like 4 over Delta-like 1

Andrawes M, et al., 288(35), 25477-25489, J Biol Chem, 2013

This report utilizes signaling assays and biochemical studies of purified recombinant ligand and receptor molecules to investigate the differences in signaling behavior and intrinsic affinity between Notch1-Dll1 and Notch1-Dll4 complexes. Notch-ligand binding affinities were determined using the BLItz system. Biotinylated EGF6-15 region of Notch1 was immobilized on Streptavidin biosensors and bound to varying concentrations of Delta-like ligands Dll1(1-5) or Dll4(1-5).

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