Interaction of AIM with Insulin-Like Growth Factor-Binding Protein-4

You Q, et al., Epub ahead of print, Int J Mol Med, 2015

This investigation has uncovered first ever Insulin-like growth factor-binding protein (IGFBP) binding partners to apoptosis inhibitor of macrophages (AIM) by co-immunoprecipitation (co-IP) and Bio-Layer Interferometry (BLI). The interaction between AIM and IGFBP-4 was evaluated by a Pall ForteBio Octet RED system equipped with Super-Streptavidin (SSA) Biosensor probes. Biotinylated IGFBP-4 captured on to SSA biosensors allowed screening against a dilution series of AIM. Authors believe that these findings will provide clues on the mechanism of action for AIM-mediated cell survival.

Read More