Integrin Binding Human Antibody Constant Domains - Probing the C-terminal Structural Loops for Grafting the RGD Motif

Traxlmayr, M.W., et al., 155(2), 193-202, J Biotechnology, 2011

In this paper, C-terminal structural loops in the CH3 domains of homodimeric IgG1-Fc were functionalized to form integrin-binding sites. The impact of this structural change upon IgG1-Fc structural integrity, thermal stability and binding to the ligands Protein A, CD16 and FcRn was probed. Protein A binding for each IgG1-Fc variant was characterized using the Octet QK system with Protein A biosensors.

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