In Vitro Analyses of the Dysregulated R206H ALK2 Kinase-FKBP12 Interaction Associated with Heterotopic Ossification in FOP

Groppe, J.C., et al., 194 (2-4), 291-295 , Cells Tissues Organs, 2011

A single arginine-for-histidine substitution in ALK2 kinase is linked to classic fibrodysplasia ossificans progressiva (FOP). Interaction analyses with purified wild-type and R206H ALK2 kinase to FKBP12 inhibitory protein was performed using the Octet QK system. Biotinylated FKBP12 protein was immobilized on Streptavidin biosensors, and then interacted into wild-type or R206H ALK2 kinase. The R206H substitution in ALK2 kinase resulted in diminished binding to FKBP12.

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