Imperfect Centered miRNA Binding Sites are Common and Can Mediate Repression of Target mRNAs

Martin HC, et al., 15(3):R51, Genome Biol, 2014

Structural and functional studies were carried out to identify the targets of 10 human microRNAs (miRNAs), which are small non-coding RNAs that regulate protein-coding genes in association with the RNA-induced silencing complex (RISC) and have a key role in transcriptional regulation. Biotin pull-down experiments showed that the highly conserved central regions of the different miRNAs have a role in regulating the binding/interaction with mRNAs and could explain the evolutionary conservation for this region and examining the mechanism of transcriptional regulation and evaluating disease-causing mRNA variants. The Octet RED system was used to examine the association and disassociation profiles of the miRNAs and Streptavidin biosensors were used.

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