Immunodominant Protein MIP_05962 from Mycobacterium Indicus Pranii Displays Chaperone Activity

Sharma A, et al., 284(9):1338-1354, FEBS J, 2017

Mycobacterium indicus pranii (MIP) is a nonpathogenic, saprophytic, cultivable organism and it has attracted attention as a potential intervention against many diseases including tuberculosis. Reported herein is the biophysical and biochemical characterization of an immunodominant MIP protein, MIP_05962 as a molecular chaperone. The interaction of MIP_05962 protein with one of its substrate proteins, citrate synthase (CS) was studied by using the Bio-Layer Interferometry (BLI) platform. A Pall ForteBio BLItz instrument equipped with his-tagged MIP_05962 protein immobilized Ni-NTA Biosensor probes was used to perform all BLI experiments. CS (native or non-native) was allowed to interact with MIP_05962 captured biosensor tips in an equimolar ratio. BLI data revealed that MIP_05962 binds only to non-native proteins. Overall results of this investigation confirms MIP_05962 as a molecular chaperone.

Read More