Identifying Low-Level Sequence Variants via Next Generation Sequencing to Aid Stable CHO Cell Line Screening

Zhang S, et al., doi: 10.1002/btpr.2119, Biotechnol Prog, 2015

This article describes for the first time, the application of transcriptome sequencing (RNAseq) in an IgG1 monoclonal antibody (mAb) cell line development (CLD) process to assist stable Chinese hamster ovary (CHO) cell line screening, by identifying low-level sequence variants. Batch titers were measured using a Pall ForteBio Octet instrument. Results suggest that RNAseq is a rapid and highly sensitive technique to detect genetic mutation de novo, and it is useful for cell line screening at various stages of CLD.

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