Identification of Human Neutralizing Antibodies Against MERS-CoV and their role in Virus Adaptive Evolution

Tang XC, et al., doi: 10.1073/pnas.1402074111, Proc Natl Acad Sci U S A, 2014

In this report, the authors screened a human Ab-phage library using a novel panning strategy to identify seven neutralizing antibodies against Middle East Respiratory Syndrome coronavirus (MERS-CoV) which causes severe respiratory disease with ~43% mortality. They further defined key residues that impacted receptor binding, viral fitness and had neutralizing effects leading to new possibilities for human mAb-based immunotherapies. Binding studies were performed on the Oct RED96 using Anti-FLAG biosensors.

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