Identification of Human Monoclonal Antibodies Specific for Human SOD1 Recognizing Distinct Epitopes and Forms of SOD1

Broering, TJ, et al., 8(4):e61210, PLoS One, 2013

Misfolded SOD1 may be implicated in the pathogenesis of amyotrophic lateral sclerosis (ALS). The authors generated and characterized a panel of fully human mAbs directed against human SOD1. Relative avidity and cross-competition experiments were performed on the Octet system equipped with Anti-Human Fc Capture (AHC) biosensors. The mAbs were loaded onto the AHC biosensors for avidity measurements. For cross-competition assays, a mAb first was loaded onto the AHC Biosensor, followed by sequential incubation with recombinant SOD1 and the competing mAb.

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