Identification of Broadly Protective Human Antibodies to Pseudomonas Aeruginosa Exopolysaccharide Psl by Phenotypic Screening

Digiandomenico, A., et al., 209(7), 1273-87, J Experimental Medicine, 2012

Whole P. aeruginosa cells and single-chain variable fragment phage libraries derived from patients were employed to select for monoclonal antibodies against P. aeruginosa infection. Affinity and epitope specificity for the mAbs against the P. aeruginosa exopolysaccharide PsI were analyzed using the Octet 384 instrument. Crude PsI preparations were immobilized onto APS biosensors for these studies. The authors also present novel carbohydrate capture experiments using the Octet system.

Read More