Identification of a Common Epitope Between Enterovirus 71 and Human MED25 Proteins Which May Explain Virus-Associated Neurological Disease

Fan P, et al., 27;7(4):1558-77, Viruses, 2015

This article describes for the first time, the identification of a common epitope (PPGAPKP) of human mediator complex subunit 25 (MED25) and Enterovirus 71 VP1. A Pall ForteBio Octet RED96 system was used to determine the affinity of the monoclonal antibody (2H2) to both MED25 and VP1. Biotinylated 2H2 mAb immobilized onto Streptavidin Biosensor probes was screened against a dilution series of the proteins.

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