Human B-cell Ontogeny in Humanized NOD/SCID γcnull Mice Generates a Diverse Yet Auto/Poly-and HIV-1-reactive Antibody Repertoire

Chang, H., et al., 13(5), 399-410, Genes and Immunity, 2012

In this study, analysis of V-H and V-kappa gene arrangements in humanized NOD/SCID mice-derived single human B cells sorted at different developmental stages was performed. The Octet RED system was used for affininty measurements of hNSG-derived scFvFcs to HIV-1gp140. Abs were loaded on Protein A biosensors and exposed to recombinant HIV-1gp140 solutions to determine kinetic binding constants.

Read More