HLA-DO Acts as a Substrate Mimic to Inhibit HLA-DM by a Competitive Mechanism

Guce, A I, et al., 20(1), 90-8, Nat Struct Mol Biol, 2013

This study investigates how the nonclassical MHCII protein HLA-DO modulates the function of another nonclassical MHCII protein, HLA-DM. Binding studies on the Octet QK system showed that HLA-DO acts as a competitive inhibitor of HLA-DM. The effects of several mutations of HLA-DM at the putative MHCII interaction surface also were evaluated using the Octet system. Biotinylated proteins and Streptavidin biosensors were used for interaction experiments.

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