High-Throughput mAb Expression and Purification Platform Based on Transient CHO

Barnard GC, Hougland M, Rajendra Y, 31(1):239-47, Biotechnol Prog, 2015

This article describes a coupled approach based on high-throughput transient transfection process involving CHO cells and a high-throughput protein A purification process, which could be used as a mAb drug discovery tool. Antibody titers were measured using a Pall ForteBio Octet QK system equipped with Protein A biosensor probes in the Quantitation mode. Calibration curves generated involve appropriate mAb standards.

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