High Throughput Cross-Interaction Measures for Human IgG1 Antibodies Correlate with Clearance Rates in Mice

Kelly R, et al., 7(4):770-7, MAbs, 2015

The development of monoclonal antibodies (mAbs) into drugs can often be difficult due to developability issues. Therefore implementing early stage developability screening assays is important to minimize downstream risks. In this study the authors have selected a panel of 16 monoclonal antibodies (mAbs) exhibiting different developability profiles, and investigated their later stage behavior from expression titer to accelerated stability and pharmacokinetics in mice. A clone self-interaction by Bio-Layer Interferometry (CSI-BLI) assay was perfomed using a Pall ForteBio Octet HTX system equipped with AHQ biosensors. The results indicate that the assays tested could be used to screen panels of lead antibodies at a reasonably early stage in the drug discovery process.

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