High Temperature SELMA: Evolution of DNA-Supported Oligomannose Clusters Which Are Tightly Recognized by HIV bnAb 2G12

Temme JS, et al., 136(5):1726-9, J Am Chem Soc, 2014

SELMA (SELection with Modified Aptamers) was performed using a modified protocol at 37°C to screen and identify glycocluster clones that exhibit strong target recognition to the neutralizing HIV antibody 2G12. Interestingly, the binding affinity of the synthetic glycocluster constructs are as strong as the HIV envelope protein gp120 yet contain just 3-4 glycans. The binding kinetics of the screened constructs was performed on the BLItz system, using Streptavidin biosensors.

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