High-end pH-controlled Delivery of Glucose Effectively Suppresses Lactate Accumulation in CHO Fed-batch Cultures

Gagnon, M., et al., 108(6), 1328-1337, Biotechnology and Bioengineering, 2011

A method was developed to control lactate accumulation in suspension cultures of CHO cells, based on the culture's pH. When applied to a 12 day fed-batch process with intial high cell densities in a concentrated medium, final titers were almost doubled relative to the previous process. The Octet system was used for titer determinations for various proteins in CHO crude supernatants.

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