Heat Shock Protein 90 Mediates the Apoptosis and Autophage in Nicotinic-Mycoepoxydiene-Treated HeLa Cells

Sun Y, et al., 47(6):451-8, Acta Biochim Biophys Sin (Shanghai), 2015

This article characterizes the interaction between Heat shock protein 90 (Hsp90) and its inhibitor nicotinicmycoepoxydiene (NMD). A Pall ForteBio Octet RED system equipped with Super-Streptavidin (SSA) biosensors was used to capture biotinylated Hsp90 binding to NMD. The KD value obtained from the Octet RED system is comparable to that obtained from a Fluorescent assay. Overall results suggest a novel mechanism pertaining to Hsp90 inhibition by NMD.

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