GlycoDelete Engineering of Mammalian Cells Simplifies N-glycosylation of Recombinant Proteins

Meuris L, et al., doi: 10.1038/nbt.2885, Nat Biotechnol, 2014

This report described a new methodology called GlycoDelete, a glycoengineering strategy used as an approach to solve the issues of N-glycosylation heterogeneity in mammalian cell-based glycoprotein production. The goal of the protocol is to produce more consistent therapeutic protein preparations in process development settings. Real-time binding was performed on an Octet RED96 system. Anti-penta his biosensors were used.

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