Global Identification of O-GlcNAc Transferase (OGT) Interactors by a Human Proteome Microarray and the Construction of an OGT Interactome

Deng RP, et al., doi: 10.1002/pmic.201300144, Proteomics, 2014

In this report, the authors screened a human proteome microarray to identify proteins that interact with OGT (O-GlcNac transferase) the key enzyme responsible for O-GlcNacylation and uncovered 25 novel targets of OGT that are known to play important roles in protein transportation/localization and transcriptional regulation providing greater insight in the role of OGT. Kinetic binding interactions were measured on the Octet system and Streptavidin biosensors were used.

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