Functional and Structural Studies of the Nucleotide Excision Repair Helicase XPD Suggest a Polarity for DNA Translocation

Kuper, J., et al., 31(2), 494-502, The EMBO Journal, 2011

The subject of this study is the XPD, a critical protein in transcription and nucleotide excision repair. The authors determine the structure of an XPD protein complexed with a short DNA fragment, and then perform mutational and biochemical analysis of XPD to define details of the XPD-ssDNA interaction. The Octet RED platform was used to determine protein-ssDNA binding constants for wild-type XPD and a panel of variants harboring point mutations. Biotinylated ssDNA was immobilized onto Streptavidin biosensors for these studies.

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