Flexible Label-free Quantitative Assay for Antibodies to Influenza Virus Hemagglutinins

Carney, P.J., et al., 17(9), 1407-1416, Clinical and Vaccine Immunology, 2010

This article reports the use of a cell-free and label-free flu antibody biosensor assay developed on the Octet RED platform for influenza research and diagnostics that utilizes recombinant hemagglutinin (HA) in conjunction with BLI to measure biomolecular interactions between the HA and specific anti-HA antibodies or sialylated ligands. The authors evaluated the assay to determine anti-HA antibody binding activity in serum or plasma to assess vaccine-induced humoral responses. They note that the Octet platform assay could be used in global surveillance laboratories since preliminary tests on desiccated HA-loaded biosensors showed no loss of activity after >2 months in storage at room temperature, indicating that the same reagent lots could be used in different laboratories to minimize interlaboratory assay fluctuation. They also note that the Octet systems offer a robust platform for influenza surveillance activities.

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