Five Birds, One Stone: Neutralization of α-Hemolysin and 4 bi-Component Leukocidins of Staphylococcus aureus with a Single Human Monoclonal Antibody

Rouha H, et al., 7(1):243-54, MAbs, 2015

This article describes the discovery of human mAbs with an ability to cross neutralize Alpha-hemolysin (Hla) and multiple leukocidins. Given the high level of pathogenesis of Staphylococcus aureus, the authors used murine models of S. aureus pneumonia and sepsis to make their case. A Pall ForteBio Octet RED96 system was used to determine binding affinities, cross reactivity, specificity (epitope binning), and the binding inhibition by phosphocholine. In these experiments, the biotinylated antigen or the antibody was captured using either Streptavidin or Anti-Human Capture (AHC) biosensor probes and screened against the antibody, the antigen, or the toxin using the Octet system.

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