Ferredoxin Competes with Bacterial Frataxin in Binding to the Desulfurase IscS

Yan R, et al., 288(34), 24777-87, J Biol Chem, 2013

The authors have characterized the interactions of the bacterial desulferase IscS and the specialized ferredoxin Fdx using a combination of biophysical tools and mutagenesis. The Octet RED system was used for binding experiments. Biotinylated holo-Fdx was loaded onto Streptavidin biosensors to quantify the affinity of the IscS-Fdx interaction and the effect of Fdx on Fe-S cluster assembly.

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