Expression of High-Affinity Human Antibody Fragments in Bacteria

R Rouet, et al., 7(2), 364-373, Nature Protocols, 2012

General protocols are presented with trouble shooting tips for the expression of human antibody fragments in E. coli. The authors discuss the use of BLI and SPR for the determination of antibody fragment-antigen binding affinitites, clone ranking, and off-rate selection, although no data are shown.

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