Existence of Separate Domains in Lysin PlyG for Recognizing Bacillus anthracis Spores and Vegetative Cells

H. Yang, et al., 56(10), 5031-5039, Antimicrob Agents Chemother, 2012

A 60 amino acid domain of the potent anti-microbial bacteriophage lysin plyG was shown to selectively recognize Bacillus anthracis spores but not vegetative cells. This discovery has implications in identfying new biomarkers for identfiying Bacillus anthracis spores and in developing plyG as a preventive antibiotic to reduce the threat of anthrax in suspected exposures to spores. The Octet system was used to assess binding of various protein domains to biotinylated Bacillus anthracis spores immobilized on Streptavidin biosensors.

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