Estimation of Raw Material Performance in Mammalian Cell Culture Using Near infrared Spectra Combined with Chemometrics Approaches

Lee, H.W, et al., 28(3), 824-832, Biotechnology Progress, 2012

Soy hydrolysate is a commonly used supplement in mammalian cell cultures, and often exhibits considerable variability in its growth-promoting and production- enhancing activities due to the compositional variability. In this study, both near-infrared spectra and bioassays of multiple soy lots were analyzed using the approaches of multivariate data analysis. Cultures were harvested after 7 days of incubation for IgG titer analysis using an Octet QK system. The Octet system titer values were used as one of the quantifiable culture performance indices throughout the study.

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