Publications

Estimation of Raw Material Performance in Mammalian Cell Culture Using Near infrared Spectra Combined with Chemometrics Approaches

Lee, H.W, et al., 28(3), 824-832, Biotechnology Progress, 2012

Soy hydrolysate is a commonly used supplement in mammalian cell cultures, and often exhibits considerable variability in its growth-promoting and production- enhancing activities due to the compositional variability. In this study, both near-infrared spectra and bioassays of multiple soy lots were analyzed using the approaches of multivariate data analysis. Cultures were harvested after 7 days of incubation for IgG titer analysis using an Octet QK system. The Octet system titer values were used as one of the quantifiable culture performance indices throughout the study.

Read More