Enzyme Kinetics and Interaction Studies for Human JNK1[beta]1 and Substrates ATF2 And cJun

Figuera-Losada, M.; LoGrasso, P.V., 287(16), 13291-13302, J Biological Chemistry, 2012

The authors report on the binding mechanism of c-Jun N-terminal kinase (JNK) with transcription factors ATF2 and c-Jun. Interaction affinity values for these proteins are reported. Both the phosphorylation state of the kinase and the presence of ATP significantly affected the kinetics of association and dissociation of JNK1 1 and its substrates, and therefore the affinity for the interactions, as elucidated by Octet RED system assay data.

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