Dynamin_SNARE Interactions Control Trans-SNARE Formation in Intracellular Membrane Fusion

Alpadi, K, et al., 4, 1704, Nat Commun, 2013

This study explores the molecular mechanism of how the dynamin-like protein Vsp1 controls membrane fusion and coordinates fusion with antagonistic fission processes. The Octet RED96 system was used to characterize the specificity and kinetics of Vsp1 binding to the SNARE protein Vam3. GST-Vam3 was loaded onto Anti-GST biosensors and incubated with various concentrations of His-Vsp1 to determine kinetic rate constants and the equilibrium dissociation constant.

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