During Cytochrome c Maturation CcmI Chaperones Class I Apocytochromes Until the Formation of Their b-Type Cytochrome Intermediates

Verissimo A, Shroff N, Daldal F, 290(27):16989-7003., J Biol Chem, 2015

The authors investigated the heme effects on interactions involving CcmI, apoCcmE, and different c-type apocytochromes in order to understand how R. capsulatus Ccm System I matures structurally dissimilar c-type cytochromes. The protein-protein interactions were monitored by a Pall ForteBio Octet RED96 system equipped with Streptavidin coated biosensors. Biotinylated c-type apocytochromes captured on to these SA biosensors were incubated with a dilution series of CcmI or apoCcmE.

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