Domain Activities of PapC Usher Reveal the Mechanism of Action of an Escherichia coli Molecular Machine

Volkan, E, et al., 109 (24), 9563-9568, Proc Natl Acad Sci USA, 2012

The Octet RED system was used to investigate the chaperone-usher pathway used by Gram-negative pathogens. Binding interactions of the periplasmic PapC functional domains NTD, CTD2, and Plug were studied to gain insight into the molecular basis of usher-catalyzed pilus biogenesis and how these domains function as a molecular machine in E.coli. All interaction experiments were carried out by capturing the biotinylated ligands (PapC or chaperone-subunit complexes) onto the Super Streptavidin biosensors and then dipping into specific binding partners at various concentrations.

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