Directed Evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process

Houlihan G, et al., pii: gzv029 Epub ahead of print, Protein Eng Des Sel, 2015

The application of SNAP display technology for in vitro evolution of Designed Ankyrin Repeat Proteins (DARPins) targeting HER2 is reported. The KD values of the mutant DARPins were investigated against biotinylated HER2 using a Pall ForteBio Octet RED system equipped with Streptavidin Biosensor probes.

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