Development of a VHH-Based Erythropoietin Quantification Assay

Kol S, et al., 57(8):692-700, Mol Biotechnol, 2015

The development and implementation of a robust assay for erythropoietin (EPO) quantification using a Pall ForteBio Octet RED96 system is reported. Traditionally, this type of quantification is performed with either ELISA or HPLC. However, the current method is capable of detecting EPO in Chinese hamster ovary cell supernatants with better sensitivity in a pH-dependent manner. The capture antibody used in this assay development is a camelid single-domain antibody fragment that is specific for EPO. A Pall ForteBio Octet RED96 system equipped with Streptavidin biosensors was used to capture biotinylated anti-EPO VHH or anti-EPO IgG ligands. The assays carried out under various conditions suggest a much broader applicability of the BLI platform.

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