Crystal Structure of the Bloom's Syndrome Helicase Indicates a Role for the HRDC Domain in Conformational Changes

Newman J, et al., 43(10):5221-35, Nucleic Acids Res, 2015

The crystal structure of Bloom's Syndrome Helicase (BLM) in complex with adenosine diphosphate (ADP) and a llama antibody single domain fragment (nanobody) as well as two other structures showing BLM in complex with DNA are reported. The data suggest tight packing between helicase and RNase D C-terminal (HRDC) and the two RecA like domains D1 and D2. A Pall ForteBio Octet RED384 system equipped with Streptavidin biosensors was used to capture biotinylated HRDC and screened against a dilution series of RecA.

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