Crystal Structure, Conformational Fixation and Entry-Related Interactions of Mature Ligand-Free HIV-1 Env

Do Kwon Y, et al., 22(7):522-31, Nat Struct Mol Biol, 2015

The crystal structure of conformationally fixed ligand free HIV-1 Env trimer and its receptor interactions are reported. In order to investigate the physical stability of the prefusion, the proteins were subjected to a variety of pharmaceutically relevant stress conditions such as high temperature, repeated freeze thaw cycles, and extreme pH. The fraction of binding to V1V2-antibody (CAP256-VRC26.09) retained after stress treatment was investigated with a Pall ForteBio Octet HTX system.

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