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Conditional Trimerization and Lytic Activity of HIV-1 gp41 Variants Containing the Membrane-Associated Segments

Dai Z, et al., 54(8):1589-99, Biochemistry, 2015

This report has revealed the fact that HIV-1 gp41 subunit can disintegrate into a monomeric form as a result of changes in pH in membrane environments. It also suggests that the monomeric form still remains active and represents a folded form. A Pall ForteBio BLItz system was employed to measure affinities between gp41 constructs and MAbs. The antibodies were captured using Anti-human Fc capture biosensors (at pH 7 or pH 4) and dipped into varying concentrations of gp41.

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