Characterization of Protective Immune Response Elicited by a Trimeric Envelope Protein from an Indian Clade C HIV-1 Isolate in Rhesus Macaques

Menon V, et al., doi: 10.1111/jmp.12178, J Med Primatol, 2015

This is an extension to a previous investigation carried out by the authors to demonstrate the immunogenic nature of gp145 in rhesus macaques. Trimeric gp145 binding to sCD4 and several HIV-1 monoclonal antibodies were evaluated by Bio-Layer Interferometry(BLI). A Pall ForteBio Octet RED system equipped with Streptavidin biosensors was used to capture biotinylated 93IN101 gp145. The sensors were dipped into serial dilutions of sCD4 or IgG. The results suggest a strong antibody response triggered by Env proteins in macaques.

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