BolA Is a Transcriptional Switch That Turns Off Motility and Turns on Biofilm Development

Dressaire C, et al, 6(1):e02352-14, MBio, 2015

This investigation has focused on target interaction and global effects of BolA, widespread gene product of bolA, initially identified in E. coli that promotes round morphology upon overexpresssion. With the help of ChIP-seq and transcriptomic analysis, the authors revealed BolA involvement in the regulation of flagellar and curli biosynthesis pathways. The affinity measurements of BolA with biotinylated DNA fragments were achieved using a Pall ForteBio BLItz system equipped with Streptavidin biosensors.

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