Assembly of Cellulases with Synthetic Protein Scaffolds In Vitro

Yu T, et al., 2:16, Bioresources and Bioprocessing, 2015

The construction of an artificial protein scaffold and its applicability to assembly of a complex comprised of three-enzyme cellulases is described. The functionality of such protein scaffolds was established with an apparent increase in enzymatic activity. Binding interactions between protein candidates were estimated by a Pall ForteBio Octet QKe system equipped with Streptavidin Biosensor probes.

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