Aptamers as a Replacement for Antibodies in Enzyme-Linked Immunosorbent Assay

Toh S, et al., 64:392-403, Biosens Bioelectron, 2015

This is a comprehensive review on the application of aptamers in assays where antibodies are originally used. Aptamers are nucleic acid based affinity agents with several advantages as compared to antibodies such as smaller size, susceptibility to modifications, very low production costs, ability to generate against a variety of target molecules. The review elaborates on several different ELISA-inspired approaches and platforms collectively known as ELASA (enzyme-linked apta-sorbent assay). Authors also highlighted the advantages of using a BLI platform to carry out ELASA assays by using a BLI-ELISA study.

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